shrna constructs based prna-u6.1 Search Results


control sirna (prna-u6.1/neo/siluc  (GenScript corporation)
90
GenScript corporation control sirna (prna-u6.1/neo/siluc
Control Sirna (Prna U6.1/Neo/Siluc, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+constructs+based+prna-u6%2E1/prna+u6+1+neo/pmc00419909-187-9-15
Average 90 stars, based on 1 article reviews
control sirna (prna-u6.1/neo/siluc - by Bioz Stars, 2026-08
90/100 stars
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prnau6 1 vector  (New England Biolabs)
99
New England Biolabs prnau6 1 vector
(A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty <t>pRNAU6.1</t> (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.
Prnau6 1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+constructs+based+prna-u6%2E1/HindIII/pmc03470565-130-5-14
Average 99 stars, based on 1 article reviews
prnau6 1 vector - by Bioz Stars, 2026-08
99/100 stars
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shrna constructs and cap1 knockdown shrna constructs based on a prna-u6.1/neo vector  (GenScript corporation)
90
GenScript corporation shrna constructs and cap1 knockdown shrna constructs based on a prna-u6.1/neo vector
(A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty <t>pRNAU6.1</t> (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.
Shrna Constructs And Cap1 Knockdown Shrna Constructs Based On A Prna U6.1/Neo Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+constructs+based+prna-u6%2E1/shrna+constructs/pmc02661149-374-25-29
Average 90 stars, based on 1 article reviews
shrna constructs and cap1 knockdown shrna constructs based on a prna-u6.1/neo vector - by Bioz Stars, 2026-08
90/100 stars
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prna-u6.1/neo-hmgb1  (GenScript corporation)
90
GenScript corporation prna-u6.1/neo-hmgb1
(A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty <t>pRNAU6.1</t> (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.
Prna U6.1/Neo Hmgb1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+constructs+based+prna-u6%2E1/small+interfering+rna++sirna+/pm25625772-35-16-32
Average 90 stars, based on 1 article reviews
prna-u6.1/neo-hmgb1 - by Bioz Stars, 2026-08
90/100 stars
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prna u6 1 vector  (Addgene inc)
93
Addgene inc prna u6 1 vector
(A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty <t>pRNAU6.1</t> (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.
Prna U6 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+constructs+based+prna-u6%2E1/pRNA-U6-let7+sponge+(Plasmid+%2335664)/pmc07477046-35-36-38
Average 93 stars, based on 1 article reviews
prna u6 1 vector - by Bioz Stars, 2026-08
93/100 stars
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prnatu6.1 hdac3 shrna4neg (plasmid #112273)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prnatu6.1 hdac3 shrna1 (plasmid #112249)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prnatu6.1 hdac3 shrna3 (plasmid #112272)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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prnatu6.1 hdac3 shrna2 (plasmid #112271)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


(A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty pRNAU6.1 (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.

Journal: PLoS ONE

Article Title: Gene-Gene Interaction and Functional Impact of Polymorphisms on Innate Immune Genes in Controlling Plasmodium falciparum Blood Infection Level

doi: 10.1371/journal.pone.0046441

Figure Lengend Snippet: (A) Schematic representation of reporter gene constructs for IL12B 3′UTR A and C alleles used for transfection assays. (B) Entire 3′UTR region was mapped for putative microRNA binding sites. The highlighted, boldfaced and underlined segments within IL12B sequence were the seed sequences for the miRNAs. (C) Schematic representation of the score and seed position of four miRNAs on target IL12B. (D & E) Normalized luciferase relative light units (RLU) in HepG2 cells were measured for IL12B+1188 A and C allele containing pSiCHECK2 constructs with (+) and without (−) the effect of miRNAs. Co-transfection of the empty pRNAU6.1 (+) vector with pSiCHECK2-IL12B+1188 construct was set as 100% and reductions in luciferase expression in presence of four miRNAs were measured in relation to this. hsa-miR-23a and hsa-miR-23b resulted in significant reduction in luciferase activities for both pSiCHECK2-IL12B+1188A and pSiCHECK2-IL12B+1188C constructs. Statistical significance was measured with t-test. (*) indicates the P values and percentage reduction to be statistically significant.

Article Snippet: The amplicons were inserted into pRNAU6.1 vector by double digestion with BamHI and HindIII (New England Biolabs, UK Ltd.).

Techniques: Construct, Transfection, Binding Assay, Sequencing, Luciferase, Cotransfection, Plasmid Preparation, Expressing